ProtectONE was tested in 2012 by the Medical Microbiology and Infectious Diseases Laboratory, University of Camerino (ranked as the best Italian university by Guida Censis Repubblica 2011 and 2012). The results clearly showed that the test dogs, which already had a tick or flea, TICKLESS prevented any worsening of the infestation and in some cases reduced the infestations. The test also showed that in dogs that were not infested prior to the start of the study, TICKLESS prevented any infestation.